pMal-E4rGel
(Plasmid
#51280)
-
PurposeExpresses a fibronectin fusion with gelonin targeting EGFR in bacteria
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 51280 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMal
-
Backbone manufacturerNEB
- Total vector size (bp) 7800
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL1 Blue
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameE4rGel
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMal-E4rGel was a gift from Dane Wittrup (Addgene plasmid # 51280 ; http://n2t.net/addgene:51280 ; RRID:Addgene_51280) -
For your References section:
Convergent potency of internalized gelonin immunotoxins across varied cell lines, antigens, and targeting moieties. Pirie CM, Hackel BJ, Rosenblum MG, Wittrup KD. J Biol Chem. 2011 Feb 11;286(6):4165-72. doi: 10.1074/jbc.M110.186973. Epub 2010 Dec 7. 10.1074/jbc.M110.186973 PubMed 21138845