pmRFP-N1 human cofilin R21Q
(Plasmid #51279)

• Purpose: expresses human cofilin R21Q mutant fused to mRFP in mammalian cells
• Depositing Lab: James Bamburg
• Publication: Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi: 10.1371/journal.pone.0083609.

Backbone

• Vector backbone: pmRFP-N1
• Backbone manufacturer: R.Y. Tsien, PNAS 2002 v99 pp7877–7882
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cofilin 1
• Alt name: CFL1
• Species: H. sapiens (human)
• Insert Size (bp): 500
• Mutation: R21Q, non-rod-forming mutation that can be used to track cofilin-actin rods in vivo without causing cofilin overexpression artifacts
• Entrez Gene: CFL1 (a.k.a. CFL, HEL-S-15, cofilin)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: mRFP1-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This cofilin plasmid contains some silent mutations to make it resistant to an siRNA used in the Bamburg lab. The mutations change the wildtype sequence (AAG TCT TCA ACG CCA GAG GAG) into AAG AGC TCA ACC CCA GAG GAG.