Skip to main content
Addgene

pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A
(Plasmid #51257)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51257 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEF5-FRT-DEST
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression ; Flpin system
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gli2
  • Species
    M. musculus (mouse)
  • Mutation
    amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala
  • GenBank ID
  • Entrez Gene
    Gli2
  • Promoter EF1a
  • Tag / Fusion Protein
    • 3xHA-TEV (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A was a gift from Rajat Rohatgi (Addgene plasmid # 51257 ; http://n2t.net/addgene:51257 ; RRID:Addgene_51257)

  • For your References section:

    Gli Protein Activity Is Controlled by Multisite Phosphorylation in Vertebrate Hedgehog Signaling. Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, Rohatgi R. Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 10.1016/j.celrep.2013.12.003. 10.1016/j.celrep.2013.12.003 PubMed 24373970
Related items:
Commonly requested with: