pEF5/FRT/DEST-3xHA/mGli2 P5-6D
(Plasmid
#51254)
-
PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51254 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEF5-FRT-DEST
-
Backbone manufacturerInvitrogen
-
Vector typeMammalian Expression ; Flpin system
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameGli2
-
SpeciesM. musculus (mouse)
-
Mutationamino acids 923,956 mutated to Asp
-
GenBank ID
-
Entrez GeneGli2
-
Tag
/ Fusion Protein
- 3xHA-TEV (N terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEF5/FRT/DEST-3xHA/mGli2 P5-6D was a gift from Rajat Rohatgi (Addgene plasmid # 51254 ; http://n2t.net/addgene:51254 ; RRID:Addgene_51254) -
For your References section:
Gli Protein Activity Is Controlled by Multisite Phosphorylation in Vertebrate Hedgehog Signaling. Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, Rohatgi R. Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 10.1016/j.celrep.2013.12.003. 10.1016/j.celrep.2013.12.003 PubMed 24373970