pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A Sequences (3)

Addgene Sequences:

Partial (3)

Partial Sequences from Addgene (3)

Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence.

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GenBank

SnapGene

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> T7 5'-TAATACGACTCACTATAGGG-3' GCTCGGATCCaCTAGTCCaGTGTGGTGGAATTCTGCAGATATCAACAAGTTTGTACAAAAAAGCAGGCTG GCGCCGGAACCAATTCAGTCGACATGTACCCATACGATGTTCCAGATTACGCTTATCCTTATGACGTACC TGACTATGCATACCCTTATGATGTACCAGACTACGCTGGCGCGGTACCAAGCGGAGGAAGCGAGAATCTT TATTTTCAGGGCACTGGAGGAACGAATTCAAGGATGGAGGCCCAGGCCCACAGCTCTACGGCGACTGAGA GGAAGAAAGCTGAAAATTCCATTGGGAAATGTCCCACGAGAACAGATGTCAGCGAGAAGGCCGTGGCCTC TAGTACCACTTCCAATGAGGATGAAAGTCCTGGACAGATCTATCACCGAGAGAGAAGAAACGCAATCACT ATGCAGCCTCAGAGTGTGCAGGGTCTCAACAAAATCAGTGAGGAGCCCTCGACGTCTAGTGATGAGAGGG CCTCGCTGATCAAGAAAGAGATCCATGGCTCTCTACCACATCTGGCGGAGCCCTCTCTCCCTTACCGTGG GACTGTGTTTGCCATGGATCCCCGGAATGGCTACATGGAGCCTCACTACCACCCTCCTCATCTTTTCCCT GCCTTCCATCCTCCTGTACCAATTGATGCCAGACATCATGAGGGCCGTTACCATTATGATCCATCTCCTA TTCCTCCATTACATGTGCCTTCTGCCTTATCTAGTAGCCCGACGTATCCAGACTTGCCCTTCATTAGGAT CTCCCCACACCGTAATCCCACTGCAGCCTCAGAGTcCCCCTTCAGCCCCCCACACCCCTACATCAA
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GenBank

SnapGene

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> BGH-rev 5'-TAGAAGGCACAGTCGAGG-3' GTACGCGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACCTTCGAACCGCGGGCCCTCTAGAC TCGAGCGGCCGCCACTGTGCTGGATATCAACCACTTTGTACAAGAAAGCTGGGTCTAGATATCTCGACTC ACTTGTCATCGTCGTCCTTGTAATCTCCGCCAGATCCTGGTGTTCCGCCACGCGTCTGCATAACTGCAAG GAACTTGCTTTCTTCTGCAAGGGAGGTCAGCAAAGAACTCATATCCCCGATAGCCATGTTGGTGGTGCCC ATGGATAGGGATGGGAATGGGAGGGATGCCCGCGGAGTGGTGAGACGGGAGGAGCTGTGGGAAAGGTTCT GAATAATACTTGGGCTCAAGGCCCCTGACATTAGGCTGGTATGGTCCCCATCATCTATGATGGCATCAAA ATCAATCTGCACACCTTCTAGGTCATGACTCTCAAAGCTGTCAACTGTGCTTGTTACCTGGTTAGCACCT GGGGAGAGTAACTCAGAGTTTTCAGTGCCATTCCCTTGGAGCAGGCAGTTGGCATCTGAGAGGGAGAACG AGCCAGCTTTCCTGTCTTGCTGACTGAAGCCCATGGTTTGGTCATAGAACTGACCGGAATAATTCTGCAC GGTAGAGCAGAGCTGCTGGGACTGACCTTGTGCCTCCATTTTGATGCTGTTCACCCTGCTGCTCTGACTC ATGTCACTGAGCTGTCCTTGTTGCAGAGTGAGGTTGCCCCTTGGCATTGCCTGACGCCTGTTACCCCCAT AGCTGGCACAGGGCTGGTAgCCCCTGATAGCTGCCATGCTTGATGGCTGGCTGCTGTTGTCCTGAgTC
Analyze Sequence

GenBank

SnapGene

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> mGli3-Fwd 5'-ACCAAATGGATGGAGCACAT-3' TGGAATGTTTCCAGACTGAACCCTATTCTACCCTCCAAAGCCCCTGCGGTATCTCCTCTCATAGGAAATG GCACACAGTCCAATAACAACTATAGCTCGGGTGGGCCCGGGACCCTCCTCCCGAGCAGAAGTGACCTGTC AGGTGTAGACTTCACAGTGCTGAACACACTCAACAGGAGAGACGCAAATACCAGCACTATCAGCTCTGCC TACCTGAGCAGCCGCAGATCCGCAGGCATCTCCCCCTGCTTTTCCAGCCGCAGGTCCGCAGAGGCGTCCC AGGCTGAAGGGCGACCCCAGAATGTGAGTGTGGCTGACTCCTACGATCCCATCTCCACAGATGCTTCAAG GAGGTCCGCAGAGGCCAGCCAGGGTGACGGGCTGCCCAGTCTGCTCAGCCTCACACCCGTGCAGCAGTAC CGCCTTAAGGCCAAGTATGCAGCTGCCACAGGTGGCCCACCACCCACACCTCTGCCCCACATGGAGAGGC TGTCACTGAAGACCAAGATGGCCTTGCTAGGGGAAGGGAGGGACTCTGGGGTGACCCTGCCTCCAGTCCA TCCTCCTCGAAGATGCGCAGATGGGGGAGGTCACACATACAGAGGGCGTCACCTGATGCCTCATGATGCA CTAGCGAACAGTGTGAGGAGAGCCGCAGACCCTGTGAGGACAGTCTCGGAGAACATGTCACTTGCCAGGG TTCAACGCTTCAGCAGCCTCAATAGCTTTAATCCTCCAAATTTGCCTCCATCGGTGGAAAAGCGTTCACT GGTCTTACAGAACTACACCCGGCAGGAGAGCAGCCAACCCCGGTACTTCCAGGCATCCCCTTGCCCTCCC AGCATCACAGAGAATGTTGCCCTGGAGGCTCTGACCATGGATGCTGATGCTAACTTGA

pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A Sequences (3)

Partial Sequences from Addgene (3)

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