pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A Sequences (3)

Addgene Sequences:

Partial (3)

Partial Sequences from Addgene (3)

Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence.

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GenBank

SnapGene

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> T7 5'-TAATACGACTCACTATAGGG-3' GAGCTCGGAtCCtCTAGTCCaGTGTGGTGGAATTCTGCAGATATCAACAAGTttgtacaaAAAAGCAGGC TGGCGCCGGAACCAATTCAGTCGACATGTACCCATACGATGTTCCAGATTACGCTTATCCTTATGACGTA CCTGACTATGCATACCCTTATGATGTACCAGACTACGCTGGCGCGGTACCAAGCGGAGGAAGCGAGAATC TTTATTTTCAGGGCACTGGAGGAACGAATTCAAGGATGGAGGCCCAGGCCCACAGCTCTACGGCGACTGA GAGGAAGAAAGCTGAAAATTCCATTGGGAAATGTCCCACGAGAACAGATGTCAGCGAGAAGGCCGTGGCC TCTAGTACCACTTCCAATGAGGATGAAAGTCCTGGACAGATCTATCACCGAGAGAGAAGAAACGCAATCA CTATGCAGCCTCAGAGTGTGCAGGGTCTCAACAAAATCAGTGAGGAGCCCTCGACGTCTAGTGATGAGAG GGCCTCGCTGATCAAGAAAGAGATCCATGGCTCTCTACCACATCTGGCGGAGCCCTCTCTCCCTTACCGT GGGACTGTGTTTGCCATGGATCCCCGGAATGGCTACATGGAGCCTCACTACCACCCTCCTCATCTTTTCC CTGCCTTCCATCCTCCTGTACCAATTGATGCCAGACATCATGAGGGCCGTTACCATTATGATCCATCTCC TATTCCTCCATTACATGTGCCTTCTGCCTTATCTAGTAGCCCGACGTATCCAGACTTGCCCTTCATTAGG ATCTCCCCACACCGTA
Analyze Sequence

GenBank

SnapGene

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> mGli3-Fwd 5'-ACCAAATGGATGGAGCACAT-3' TTTCCaAGACTGAACCCTATTCTACCCTCCAAAGCCCCTGCGGTATCTCCTCTCATAGGAAATGGCACAC AGTCCAATAACAACTATAGCTCGGGTGGGCCCGGGACCCTCCTCCCGAGCAGAAGTGACCTGTCAGGTGT AGACTTCACAGTGCTGAACACACTCAACAGGAGAGACGCAAATACCAGCACTATCAGCTCTGCCTACCTG AGCAGCCGCAGATCCGCAGGCATCTCCCCCTGCTTTTCCAGCCGCAGGTCCGCAGAGGCGTCCCAGGCTG AAGGGCGACCCCAGAATGTGAGTGTGGCTGACTCCTACGATCCCATCTCCACAGATGCTTCAAGGAGGTC CGCAGAGGCCAGCCAGGGTGACGGGCTGCCCAGTCTGCTCAGCCTCACACCCGTGCAGCAGTACCGCCTT AAGGCCAAGTATGCAGCTGCCACAGGTGGCCCACCACCCACACCTCTGCCCCACATGGAGAGGCTGAGCC TGAAGACCAAGATGGCCTTGCTAGGGGAAGGGAGGGACTCTGGGGTGACCCTGCCTCCAGTCCATCCTCC TCGAAGATGCAGTGATGGGGGAGGTCACACATACAGAGGGCGTCACCTGATGCCTCATGATGCACTAGCG AACAGTGTGAGGAGAGCCAGCGACCCTGTGAGGACAGTCTCGGAGAACATGTCACTTGCCAGGGTTCAAC GCTTCAGCAGCCTCAATAGCTTTAATCCTCCAAATTTGCCTCCATCGGTGGAAAAGCGTTCACTGGTCTT ACAGAACTACACCCGGCAGGAGAGCAGCCAACCCCGGTACTTCCAGGCATCCCCTTGCCCTCCCAGCATC ACAGAGAATGTTGCCCTGGAgGCTCTGA
Analyze Sequence

GenBank

SnapGene

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> BGH-Rev 5'-TAGAAGGCACAGTCGAGG-3' GGTAcGCGTAGAATCGAGAcCGAGGAGAGGGTTAGGGATAGGCTTACCTTCGAACCgcggGCCCTCTAGA CTCGAGCGGCCGCCACTGTGCTGgatatcaACcACTTTGTACAAGAAAGCTGGGTCTAGATATCTCGACT CACTTGTCATCGTCGTCCTTGTAATCTCCGCCAGATCCTGGTGTTCCGCCACGCGTCTGCATAACTGCAA GGAACTTGCTTTCTTCTGCAAGGGAGGTCAGCAAAGAACTCATATCCCCGATAGCCATGTTGGTGGTGCC CATGGATAGGGATGGGAATGGGAGGGATGCCCGCGGAGTGGTGAGACGGGAGGAGCTGTGGGAAAGGTTC TGAATAATACTTGGGCTCAAGGCCCCTGACATTAGGCTGGTATGGTCCCCATCATCTATGATGGCATCAA AATCAATCTGCACACCTTCTAGGTCATGACTCTCAAAGCTGTCAACTGTGCTTGTTACCTGGTTAGCACC TGGGGAGAGTAACTca

pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A Sequences (3)

Partial Sequences from Addgene (3)

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