pBEVY-U
(Plasmid #51230)

• Purpose: (Empty Backbone) bi-directional expression vector for yeast, constitutively active, URA3 selection
• Depositing Lab: Charles Miller
• Publication: Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.

Backbone

• Vector backbone: pBEVY-GU
• Backbone manufacturer: Addgene plasmid 51229
• Modifications to backbone: The GAL1,10 promoter was substituted with a constitutively active bi-directional promoter that consisted of a fusion of the GPD promoter and a short derivative of the ADH1 promoter.
• Vector type: Yeast Expression
• Promoter: GPD/ADH1
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: GPDpro_F and yADH1_F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.