pBEVY-GU
(Plasmid #51229)

• Purpose: (Empty Backbone) bi-directional expression vector for yeast, galactose inducible, URA3 selection
• Depositing Lab: Charles Miller
• Publication: Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.

Backbone

• Vector backbone: YEplac195
• Backbone manufacturer: R.D.Gietz (University of Manitoba)
• Modifications to backbone: Similar to pBEVY-GL, Addgene plasmid 51225
• Vector type: Yeast Expression
• Promoter: GAL1,10
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Gal1 and Gal10Pro_F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.