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Purpose(Empty Backbone) bi-directional expression vector for yeast, constitutively active, LEU2 selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBEVY-GL
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Backbone manufacturerAddgene plasmid 51225
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Modifications to backboneThe GAL1,10 promoter was substituted with a constitutively active bi-directional promoter that consisted of a fusion of the GPD promoter and a short derivative of the ADH1 promoter.
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Vector typeYeast Expression
- Promoter GPD/ADH1
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GPDpro_F and yADH1_F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEVY-L was a gift from Charles Miller (Addgene plasmid # 51226 ; http://n2t.net/addgene:51226 ; RRID:Addgene_51226) -
For your References section:
Assessment of aryl hydrocarbon receptor complex interactions using pBEVY plasmids: expressionvectors with bi-directional promoters for use in Saccharomyces cerevisiae. Miller CA 3rd, Martinat MA, Hyman LE. Nucleic Acids Res. 1998 Aug 1;26(15):3577-83. 10.1093/nar/26.15.3577 PubMed 9671822
Map uploaded by the depositor.
