DsRed-p150 217–548
(Plasmid #51146)

• Purpose: Inhibits dynein-dynactin interaction in mammalian cells
• Depositing Lab: Trina Schroer
• Publication: Quintyne et al J Cell Biol. 2002 Oct 28;159(2):245-54. Epub 2002 Oct 21.

Backbone

• Vector backbone: pDsRed-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DCTN1
• Alt name: p150Glued
• Species: G. gallus (chicken)
• Insert Size (bp): 997
• Mutation: Q118R, E340K
• GenBank ID: XM_015301318.1
• Entrez Gene: DCTN1 (a.k.a. Chip150)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutations Q118R and E340K were found during Addgene's quality control. The depositor noted that these mutations do NOT affect plasmid function.