pet30b-7M SrtA
(Plasmid #51141)

• Purpose: expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
• Depositing Lab: Hidde Ploegh
• Publication: unpublished (unpublished)

Backbone

• Vector backbone: pET30b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5421
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S.aureus SrtA 7M
• Alt name: Calcium independent sortase A
• Alt name: Heptamutant Sortase A
• Species: S.aureus
• Insert Size (bp): 471
• Mutation: P94R, E105K, E108Q, D160N, D165A, K190E, K196T
• Promoter: T7
• Tag / Fusion Protein:
  • 6x His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from David Liu's pentamutant sortase, includes two additional amino acid mutations which rendered it calcium independent: E105K, E108Q
• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry