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pet30b-5M SrtA
(Plasmid #51140)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51140 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S.aureus SrtA 5M
  • Alt name
    Sortase A pentamutant
  • Species
    S.aureus
  • Insert Size (bp)
    471
  • Mutation
    P94R, D160N, D165A, K190E, K196T
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Pentamutant sortase A was originally described by David R. Liu's laboratory (Harvard University) in:
A General Strategy for the Evolution of Bond-Forming Enzymes Using Yeast Display. Chen I, Dorr BM, Liu DR. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404 (PubMed ID: 21697512)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pet30b-5M SrtA was a gift from Hidde Ploegh (Addgene plasmid # 51140 ; http://n2t.net/addgene:51140 ; RRID:Addgene_51140)

  • For your References section:

    Engineered red blood cells as carriers for systemic delivery of a wide array of functional probes. Shi J, Kundrat L, Pishesha N, Bilate A, Theile C, Maruyama T, Dougan SK, Ploegh HL, Lodish HF. Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10131-6. doi: 10.1073/pnas.1409861111. Epub 2014 Jun 30. 10.1073/pnas.1409861111 PubMed 24982154
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