hUbc13
(Plasmid #51131)

• Purpose: human ubc13, E2 for synthesis k63 linked ubiquitin chain
• Depositing Lab: Cynthia Wolberger
• Publication: Wiener et al Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911.

Backbone

• Vector backbone: PETSUMO
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5800
• Total vector size (bp): 6300
• Modifications to backbone: PET32a modified SUMO is replaced the TRX tag, SUMO tag can be removed by SENP
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ubc13
• Alt name: Ube2N
• Alt name: ubiquitin-conjugating enzyme E2N
• Species: H. sapiens (human)
• Insert Size (bp): 459
• Entrez Gene: UBE2N (a.k.a. HEL-S-71, UBC13, UBCHBEN, UBCHBEN; UBC13, UbcH-ben, UbcH13)
• Promoter: T7
• Tag / Fusion Protein:
  • His6 and SUMO (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Term

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry