pBABEpuro-CRAF_S257L
(Plasmid #51125)

• Purpose: mammalian expression of wt CRAF S257L
• Depositing Lab: Matthew Meyerson
• Publication: pBABEpuro-CRAF (unpublished)

Backbone

• Vector backbone: pBABEpuro
• Backbone size w/o insert (bp): 5200
• Total vector size (bp): 7150
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRAF
• Alt name: RAF1
• Alt name: c-Raf
• Alt name: Raf-1
• Species: H. sapiens (human)
• Insert Size (bp): 1950
• Mutation: S257L
• GenBank ID: NM_002880.3
• Entrez Gene: RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry