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Addgene

pBABEpuro-ARAF_D429A
(Plasmid #51080)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51080 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABEpuro
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 7050
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARAF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Mutation
    D429A
  • GenBank ID
    NM_001654.4
  • Entrez Gene
    ARAF (a.k.a. A-RAF, ARAF1, PKS2, RAFA1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABEpuro-ARAF_D429A was a gift from Matthew Meyerson (Addgene plasmid # 51080 ; http://n2t.net/addgene:51080 ; RRID:Addgene_51080)

  • For your References section:

    Oncogenic and sorafenib-sensitive ARAF mutations in lung adenocarcinoma. Imielinski M, Greulich H, Kaplan B, Araujo L, Amann J, Horn L, Schiller J, Villalona-Calero MA, Meyerson M, Carbone DP. J Clin Invest. 2014 Apr;124(4):1582-6. doi: 10.1172/JCI72763. Epub 2014 Feb 24. 10.1172/JCI72763 PubMed 24569458
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