pBABEpuro-ARAF_S214T
(Plasmid
#51079)
-
Purposemammalian expression of ARAF S214T
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51079 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBABEpuro
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 7050
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameARAF
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1800
-
MutationS214T
-
GenBank IDNM_001654.4
-
Entrez GeneARAF (a.k.a. A-RAF, ARAF1, PKS2, RAFA1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBABEpuro-ARAF_S214T was a gift from Matthew Meyerson (Addgene plasmid # 51079 ; http://n2t.net/addgene:51079 ; RRID:Addgene_51079)
