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Purposeentry vector for cloning human TRIM9
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51032 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR/D-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2820
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTRIM9
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SpeciesH. sapiens (human)
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Entrez GeneTRIM9 (a.k.a. RNF91, SPRING)
- Promoter NA
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-D-Topo-hTRIM9 was a gift from Timothy Cox (Addgene plasmid # 51032 ; http://n2t.net/addgene:51032 ; RRID:Addgene_51032) -
For your References section:
Subclassification of the RBCC/TRIM superfamily reveals a novel motif necessary for microtubule binding. Short KM, Cox TC. J Biol Chem. 2006 Mar 31;281(13):8970-80. Epub 2006 Jan 23. 10.1074/jbc.M512755200 PubMed 16434393
Map uploaded by the depositor.
