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Purposemammalian expression of mouse Trific fused to GFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA-DEST53
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7767
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTrific
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Alt nameTrim46
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SpeciesM. musculus (mouse)
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Entrez GeneTrim46 (a.k.a. TRIFIC)
- Promoter CMV
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Tag
/ Fusion Protein
- Cycle3 GFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer GFP-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-DEST53-mTrific was a gift from Timothy Cox (Addgene plasmid # 51031 ; http://n2t.net/addgene:51031 ; RRID:Addgene_51031) -
For your References section:
Subclassification of the RBCC/TRIM superfamily reveals a novel motif necessary for microtubule binding. Short KM, Cox TC. J Biol Chem. 2006 Mar 31;281(13):8970-80. Epub 2006 Jan 23. 10.1074/jbc.M512755200 PubMed 16434393
Map uploaded by the depositor.
