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PurposeLentivector that expresses LifeAct-GFP from internal human PGK promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51010 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti.PGK:EGFP
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Backbone manufacturerL. Naldini
- Total vector size (bp) 7462
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Modifications to backboneDigested pmRFPruby-N1-Lifeact-EcoRI-BHI with Nhe1/Not1 (T4 DNAP) and ligated into RRLsin.cPPT.hPGK.eGFP.Wpre (Naldini) BamH1/Sal1/T4 DNAP/CIP vector.
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLifeAct-GFP
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Insert Size (bp)792
- Promoter human PGK
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Tag
/ Fusion Protein
- LifeAct (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (destroyed during cloning)
- 3′ cloning site Sal1 (destroyed during cloning)
- 5′ sequencing primer EGFP-N
- 3′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti.PGK.LifeAct-GFP.W was a gift from Rusty Lansford (Addgene plasmid # 51010 ; http://n2t.net/addgene:51010 ; RRID:Addgene_51010)
Map uploaded by the depositor.
