pLenti.CAG.H2B-cerFP.W.SINloxP
(Plasmid #51006)

• Purpose: Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter
• Depositing Lab: Rusty Lansford
• Publication: Lentivirus (unpublished)

Backbone

• Vector backbone: pRRLsin.cPPT.PGKp.eGFP.W
• Backbone manufacturer: L Naldini
• Total vector size (bp): 7375
• Modifications to backbone: pLenti.CAG:H2B-cerFp.W.SINloxP lentivector derives from the pRRLsin.cPPT.PGKp.eGFP.W lentivector that contains the constitutively expressed human phosphoglycerate kinase promoter (De Palma et al., 2003). Contains loxP site in the U3 region of deleted 3'LTR.
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: H2B-cerFP
• Alt name: histone 2B-ceruleanFP
• Insert Size (bp): 1116
• Promoter: CAG
• Tag / Fusion Protein:
  • histone 2B (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sma1 (not destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.