pLenti.Syn(0.5).H2B-eGFP.W
(Plasmid #51004)

• Purpose: Lentivector that expresses H2B-eGFP from neurons-specific synapsin promoter
• Depositing Lab: Rusty Lansford
• Publication: Lentivirus (unpublished)

Backbone

• Vector backbone: pLenti.Syn(0.5).eGFP
• Backbone size w/o insert (bp): 8344
• Total vector size (bp): 9655
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: H2B-EGFP
• Species: H. sapiens (human), Synthetic; Jellyfish
• Insert Size (bp): 1131
• Entrez Gene: H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
• Promoter: rat synapsin
• Tag / Fusion Protein:
  • histone2B

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (unknown if destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP-C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.