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Addgene

Gal4-p160MBP
(Plasmid #51)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMX-Gal4
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p160 Myb binding protein
  • Alt name
    p160MBP
  • Alt name
    Mybbp1a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4000
  • GenBank ID
    BC052889
  • Entrez Gene
    Mybbp1a (a.k.a. AL024407, AU019902, P160, p160MBP, p67MBP)
  • Tag / Fusion Protein
    • Gal4DBD (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer Gal4 N-term
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tom Gonda
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gal4-p160MBP was a gift from Bruce Spiegelman (Addgene plasmid # 51 ; http://n2t.net/addgene:51 ; RRID:Addgene_51)

  • For your References section:

    Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK. Fan M, Rhee J, St-Pierre J, Handschin C, Puigserver P, Lin J, Jaeger S, Erdjument-Bromage H, Tempst P, Spiegelman BM. Genes Dev 2004 Feb 1;18(3):278-89. Epub 2004 Jan 26. 10.1101/gad.1152204 PubMed 14744933
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