AAV-oChIEF-citrine
(Plasmid #50974)

• Purpose: AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR
• Depositing Lab: Roger Tsien
• Publication: Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034.

Backbone

• Vector backbone: AAV2
• Backbone size w/o insert (bp): 4599
• Total vector size (bp): 6402
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Regular growing condition.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ChIEF-citrine
• Species: Synthetic
• Insert Size (bp): 1803
• Mutation: ChIEF gene is mammalian codon optimized
• GenBank ID: KF437396
• Promoter: human synapsin promoter
• Tag / Fusion Protein:
  • citrine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • Annotated genbank sequence file
• A portion of this plasmid was derived from a plasmid made by: From Dr. Lin Tian at Janelia Farm Research Campus (currently at UC Davis)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

WPRE is inserted after the citrine before SV40 polyA.
Addgene QC NGS is unable to confirm a full 3' ITR. It is unknown what, if any, impact this may have on viral vector production.