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Addgene

AAV-flex-oChIEF-citrine
(Plasmid #50973)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50973 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    AAV2
  • Backbone size w/o insert (bp) 4599
  • Total vector size (bp) 6657
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Regular growing condition.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ChIEF-citrine
  • Species
    Synthetic
  • Insert Size (bp)
    2089
  • Mutation
    inverted and inserted between two lox sites
  • GenBank ID
    KF437396
  • Promoter human synapsin promoter
  • Tag / Fusion Protein
    • citrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    From Dr. Lin Tian at Janelia Farm Research Campus (currently at UC Davis)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

WPRE is inserted after the citrine before SV40 polyA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-flex-oChIEF-citrine was a gift from Roger Tsien (Addgene plasmid # 50973 ; http://n2t.net/addgene:50973 ; RRID:Addgene_50973)
  • For your References section:

    Characterization of engineered channelrhodopsin variants with improved properties and kinetics. Lin JY, Lin MZ, Steinbach P, Tsien RY. Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034. 10.1016/j.bpj.2008.11.034 PubMed 19254539