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Purposeflexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50973 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV2
- Backbone size w/o insert (bp) 4599
- Total vector size (bp) 6657
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsRegular growing condition.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChIEF-citrine
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SpeciesSynthetic
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Insert Size (bp)2089
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Mutationinverted and inserted between two lox sites
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GenBank IDKF437396
- Promoter human synapsin promoter
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Tag
/ Fusion Protein
- citrine (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byFrom Dr. Lin Tian at Janelia Farm Research Campus (currently at UC Davis)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
WPRE is inserted after the citrine before SV40 polyA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-flex-oChIEF-citrine was a gift from Roger Tsien (Addgene plasmid # 50973 ; http://n2t.net/addgene:50973 ; RRID:Addgene_50973) -
For your References section:
Characterization of engineered channelrhodopsin variants with improved properties and kinetics. Lin JY, Lin MZ, Steinbach P, Tsien RY. Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034. 10.1016/j.bpj.2008.11.034 PubMed 19254539