AAV-miniSOG-VAMP2-citrine
(Plasmid #50969)

• Purpose: AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct
• Depositing Lab: Roger Tsien
• Publication: Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022.

Backbone

• Vector backbone: AAV2
• Backbone size w/o insert (bp): 4575
• Total vector size (bp): 6027
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Regular growing condition.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: miniSOG-VAMP2-citrine
• Species: M. musculus (mouse); Arabdopsis thaliana
• Insert Size (bp): 1449
• GenBank ID: NM_009497
• Entrez Gene: Vamp2 (a.k.a. Syb-2, Syb2, Vamp-2, sybII)
• Promoter: human synapsin promoter
• Tags / Fusion Proteins:
  • citrine (C terminal on insert)
  • miniSOG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • Genbank annotated sequence file
• A portion of this plasmid was derived from a plasmid made by: From Dr. Lin Tian at Janelia Farm Research Campus (currently at UC Davis)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

WPRE is inserted after the citrine before SV40 polyA.