RedTrackCMV
(Plasmid #50957)

• Purpose: (Empty Backbone) A backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFP
• Depositing Lab: James Bamburg
• Publication: Bamburg lab plasmids (unpublished)

Backbone

• Vector backbone: pAdTrack-CMV
• Backbone manufacturer: Bert Vogelstein lab (Addgene Plasmid # 16405)
• Backbone size (bp): 8935
• Modifications to backbone: PCR was used to generate mRFP with XmaI on 5’ and BglII on 3’. This was ligated into AdTrackCMV in place of eGFP using AgeI and BclI.
• Vector type: Adenoviral
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Encap-F (5'-TTTGGGCGTAACCGAGTAAG-3')
• 3′ sequencing primer: pShuttle-R (5'-CACAATGCTTCCATCAAACG-3')

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry