AAV-flex-ReaChR-citrine
(Plasmid #50955)

• Purpose: flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter
• Depositing Lab: Roger Tsien
• Publication: Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1.

Backbone

• Vector backbone: AAV2
• Backbone size w/o insert (bp): 4844
• Total vector size (bp): 6630
• Modifications to backbone: two Lox sites are used to flanked the inverted ReaChR-citrine gene.
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Regular growing condition.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ReaChR-citrine
• Species: Synthetic
• Insert Size (bp): 1776
• GenBank ID: KF448069
• Promoter: human synapsin promoter
• Tag / Fusion Protein:
  • citrine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: GFP

Resource Information

• Supplemental Documents:
  • Genbank file
• A portion of this plasmid was derived from a plasmid made by: From Dr. Lin Tian at Janelia Farm Research Campus (currently at UC Davis)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

inverted ReaChR-citrine inserted between two lox sites. WPRE is used before SV40 polyA.