AAV-ReaChR-citrine
(Plasmid #50954)

• Purpose: ReaChR-citrine in AAV2 vector under human synapsin promoter
• Depositing Lab: Roger Tsien
• Publication: Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1.

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Backbone

• Vector backbone: AAV2
• Backbone size w/o insert (bp): 4589
• Total vector size (bp): 6375
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Regular growing condition.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ReaChR-citrine
• Species: Synthetic
• Insert Size (bp): 1776
• GenBank ID: KF448069
• Promoter: truncated human Synapsin promoter
• Tag / Fusion Protein:
  • citrine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • Annotated genbank sequence file
• A portion of this plasmid was derived from a plasmid made by: From Dr. Lin Tian at Janelia Farm Research Campus (currently at UC Davis)
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

WPRE is inserted after the citrine before SV40 polyA.

Please note: Based on the Addgene QC results, the AgeI site shown in the depositor's sequence is not present in this vector. There are other sequence discrepancies that do not have functional consequence.