pOTTC155
(Plasmid #50949)

• Purpose: An LV packaging vector expressing eGFP
• Depositing Lab: Brandon Harvey
• Publication: Lentiviral vectors for stable ectopic expression or knockdown of WNT proteins (unpublished)

Backbone

• Vector backbone: pLenti6.3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7700
• Total vector size (bp): 8519
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: eGFP
• Alt name: enhanced Green Fluorescent Protein
• Insert Size (bp): 720
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV_For
• 3′ sequencing primer: WPRE_R1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry