Skip to main content
Addgene

pET11a-hRanGAP1
(Plasmid #50941)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50941 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET11a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5677
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RanGTPase activating protein 1
  • Alt name
    RanGAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1764
  • GenBank ID
    NM_001278651.1
  • Entrez Gene
    RANGAP1 (a.k.a. Fug1, RANGAP, SD)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11a-hRanGAP1 was a gift from Frauke Melchior (Addgene plasmid # 50941 ; http://n2t.net/addgene:50941 ; RRID:Addgene_50941)

  • For your References section:

    The RanBP2/RanGAP1*SUMO1/Ubc9 complex is a multisubunit SUMO E3 ligase. Werner A, Flotho A, Melchior F. Mol Cell. 2012 May 11;46(3):287-98. doi: 10.1016/j.molcel.2012.02.017. Epub 2012 Mar 29. 10.1016/j.molcel.2012.02.017 PubMed 22464730
Related items:
Commonly requested with: