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Addgene

PML-3/pSG5
(Plasmid #50939)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PML-3
  • Alt name
    promyelocytic leukemia
  • Alt name
    PML
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6022
  • Mutation
    K65R, K160R, K490R (Sumo modification sites)
  • Entrez Gene
    PML (a.k.a. MYL, PP8675, RNF71, TRIM19)
  • Promoter SV40
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PML-3/pSG5 was a gift from Pier Pandolfi (Addgene plasmid # 50939 ; http://n2t.net/addgene:50939 ; RRID:Addgene_50939)

  • For your References section:

    Role of SUMO-1-modified PML in nuclear body formation. Zhong S, Muller S, Ronchetti S, Freemont PS, Dejean A, Pandolfi PP. Blood. 2000 May 1;95(9):2748-52. PubMed 10779416
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