MSCV-PIG-Cre
(Plasmid #50935)

• Purpose: retroviral expression of Cre recombinase
• Depositing Lab: Pier Pandolfi
• Publication: Chen et al Nature. 2005 Aug 4;436(7051):725-30.

Backbone

• Vector backbone: MSCV PIG, Addgene plasmid # 18751
• Backbone manufacturer: Scott Lowe
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression, Retroviral, Cre/Lox
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cre recombinase
• Insert Size (bp): 1000
• Tag / Fusion Protein:
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: MSCV

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains Puro resistance gene, IRES, and GFP downstream of PGK promoter.
The Nuclear Localization Sequence fusion Cre recombinase (NLS-Cre) was excised from pTZ-CreN vector (a gift from L. Nitschke).