pscAluYNF1
(Plasmid #50934)

• Purpose: Expresses scAluY RNA in mammalian cells.
• Depositing Lab: Katharina Strub
• Publication: Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):2874-87. doi: 10.1093/nar/gkv048. Epub 2015 Feb 19.

Backbone

• Vector backbone: pAlu-NeoTet
• Backbone manufacturer: Dewannieux,M., Esnault,C. and Heidmann,T. (2003) LINE-mediated retrotransposition of marked Alu sequences. Nat. Genet., 35, 41-4
• Backbone size w/o insert (bp): 2773
• Total vector size (bp): 2855
• Modifications to backbone: the neoTet reporter gene was removed
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: scAluYNF1
• Species: H. sapiens (human)
• Insert Size (bp): 114
• Mutation: the poly(A) linker and the right arm of the dimeric ALuYNF1 gene were removed
• Promoter: 7SL RNA promoter (pol III)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13pUC-rev
• 3′ sequencing primer: M13pUC-fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Wallace, M.R. et al. A de novo Alu insertion results in neurofibromatosis type 1. Nature 353, 864–866 (1991).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry