p14-9VN9-3A
(Plasmid
#50932)
-
PurposeExpresses human SRP14 and SRP9-3A proteins as a fusion protein in mammalian cells.
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBiFC-VN173
-
Backbone manufacturerChang-Deng Hu (Addgene Plasmid# 22010)
- Backbone size w/o insert (bp) 5216
- Total vector size (bp) 5879
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL1 Blue
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameSRP14 and SRP9
-
SpeciesH. sapiens (human)
-
Insert Size (bp)677
-
MutationAmino acids 60, 61 and 64 of SRP9 changed to alanine
-
Entrez GeneSRP14 (a.k.a. ALURBP)
-
Entrez GeneSRP9 (a.k.a. ALURBP)
- Promoter CMV
-
Tags
/ Fusion Proteins
- flag tag (N terminal on backbone)
- VN tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p14-9VN9-3A was a gift from Katharina Strub (Addgene plasmid # 50932 ; http://n2t.net/addgene:50932 ; RRID:Addgene_50932)