p14-9VN
(Plasmid #50930)

• Purpose: Expresses human SRP14 and SRP9 proteins as a fusion protein in mammalian cells.
• Depositing Lab: Katharina Strub
• Publication: Strub Lab 14-9VN plasmids (unpublished)

Backbone

• Vector backbone: pBiFC-VN173
• Backbone manufacturer: Chang-Deng Hu (Addgene Plasmid# 22010)
• Backbone size w/o insert (bp): 5216
• Total vector size (bp): 5879
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SRP14 and SRP9
• Species: H. sapiens (human)
• Insert Size (bp): 677
• Entrez Gene: SRP14 (a.k.a. ALURBP)
• Entrez Gene: SRP9 (a.k.a. ALURBP)
• Promoter: CMV
• Tags / Fusion Proteins:
  • flag tag (N terminal on backbone)
  • VN tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry