pmRFP-N1 human cofilin S3E
(Plasmid #50858)

• Purpose: expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells
• Depositing Lab: James Bamburg
• Publication: Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Backbone

• Vector backbone: pmRFP-N1
• Backbone manufacturer: R.Y. Tsien, PNAS 2002 v99 pp7877–7882
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cofilin 1 S3E
• Alt name: CFL1
• Species: H. sapiens (human)
• Insert Size (bp): 500
• Mutation: S3E psuedo-phosporylated, non-activatable
• Entrez Gene: CFL1 (a.k.a. CFL, HEL-S-15, cofilin)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: mRFP1-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a F56L mutation in the mRFP sequence. According to the depositing laboratory, this does not affect the RFP fluorescence.