pcDNA3.1 human cofilin S3E
(Plasmid #50855)

• Purpose: expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells
• Depositing Lab: James Bamburg
• Publication: Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 5912
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cofilin 1 S3E
• Alt name: CFL1
• Species: H. sapiens (human)
• Insert Size (bp): 500
• Mutation: S3E psuedo-phosporylated, non-activatable
• Entrez Gene: CFL1 (a.k.a. CFL, HEL-S-15, cofilin)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Human cofilin 1 cDNA was PCRed with a 5' primer that had an EcoRI, while the 3' primer had KpnI and EcoRV. EcoRI and KpnI were then used to ligate into pcDNA3.1- (leaving the EcoRV on the 3’ end of the insert).