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Addgene

pHR SFFVp PHAkt Cerulean
(Plasmid #50837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50837 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Lentiviral, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PHAkt
  • Alt name
    PH domain of Akt
  • Insert Size (bp)
    491
  • Entrez Gene
    AKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Tag / Fusion Protein
    • Cerulean (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR SFFVp PHAkt Cerulean was a gift from Wendell Lim (Addgene plasmid # 50837 ; http://n2t.net/addgene:50837 ; RRID:Addgene_50837)

  • For your References section:

    Light-based feedback for controlling intracellular signaling dynamics. Toettcher JE, Gong D, Lim WA, Weiner OD. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700. 10.1038/nmeth.1700 PubMed 21909100
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