pAcBac1.tR4-MbPyl
(Plasmid #50832)

• Purpose: baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
• Depositing Lab: Peter Schultz
• Publication: Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 10.1073/pnas.1309584110. Epub 2013 Jul 1.

Backbone

• Vector backbone: pAcBac
• Modifications to backbone: derived from pFastBac-Dual from Invitrogen.
• Vector type: Insect Expression ; baculoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: two-copy Pyl tRNA cassette
• Species: Synthetic
• Promoter: U6 and H1

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: MbPyl
• Alt name: pyrrolysyl-tRNA synthetase
• Species: Methanosarcina barkeri
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Gene/Insert 3

• Gene/Insert name: two copy Pyl tRNA cassette
• Species: Synthetic
• Promoter: U6 and H1

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry