pAcBac2.tR4-OMeYRS/GFP*
(Plasmid #50831)

• Purpose: baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
• Depositing Lab: Peter Schultz
• Publication: Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 10.1073/pnas.1309584110. Epub 2013 Jul 1.

Backbone

• Vector backbone: pAcBac
• Modifications to backbone: derived from pFastBac-Dual from Invitrogen.
• Vector type: Insect Expression ; baculoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: two-copy Tyr tRNA cassette
• Species: Synthetic
• Promoter: U6 and H1

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: EGFP*
• Mutation: Y39TAG
• Promoter: CAG
• Tags / Fusion Proteins:
  • WPRE (C terminal on insert)
  • Myc-6xHis (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: WPRE-R

Gene/Insert 3

• Gene/Insert name: OMeYRS
• Alt name: E. coli tyrosyl-tRNA synthetase
• Species: E. coli
• Mutation: able to charge various unnatural amino acids (UAAs) but not canonical amino acids. Y37V, D182S, F183M, D265R
• Promoter: CMV
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Gene/Insert 4

• Gene/Insert name: two copy Tyr tRNA cassette
• Species: Synthetic
• Promoter: U6 and H1

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry