miR-34aM1
(Plasmid #50827)

• Purpose: luciferase reporter for human miR-34a
• Depositing Labs: Yun Bai, Xueqing Xu
• Publication: Li et al BMC Mol Biol. 2012 Jan 31;13:4.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 6084
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miR-34A promoter M1
• Alt name: MIR34A
• Alt name: miR-34A
• Species: H. sapiens (human)
• Insert Size (bp): 1266
• Mutation: first NF-kB binding site mutated, (-539)
• Entrez Gene: MIR34A (a.k.a. MIRN34A, miRNA34A, mir-34, mir-34a)
• Promoter: miR34a
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry