EB1-tdTomato
(Plasmid #50825)

• Purpose: expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs
• Depositing Lab: Erik Dent
• Publication: Dent Lab Unpublished Plasmids (unpublished)

Backbone

• Vector backbone: tdTomato-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 6216
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EB1
• Alt name: MAPRE1
• Alt name: microtubule-associated protein, RP/EB family, member 1
• Species: M. musculus (mouse)
• Entrez Gene: Mapre1 (a.k.a. 5530600P05Rik, BIM1p, D2Ertd459e, Eb1)
• Promoter: CMV
• Tag / Fusion Protein:
  • tdTomato (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We received the EB1 construct from Yuko Mimori-Kiyosue (RIKEN, Kobe, Japan) originally and replaced the GFP with tdTomato.
There is a 4x Gly linker between the EB1 and the tdTomato.