PFA0445w-bio-His
(Plasmid
#50811)
-
PurposeExpresses enzymatically monobiotinylated full length PFA0445w
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 50811 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepTT3
-
Backbone manufacturerYves Durocher
- Backbone size w/o insert (bp) 6670
-
Modifications to backboneAddition of CD4, biotinylation sequence, 6xhis tags
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePFA0445w
-
Alt nameLCCL domain containing-protein
-
SpeciesP. falciparum
-
Insert Size (bp)3041
-
Mutationall potential N-linked glycosylation sites (N-X-S/T, where X is not proline) were systematically removed by substituting alanine for serine/threonine at these sites.
- Promoter CMV
-
Tag
/ Fusion Protein
- ratCD4d3+4,biotinylation sequence, 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBVori-F (5'-CCACTCAGTGTTGGCAAATG-3') (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bypTT3 was obtained from Yves Durocher, National Research Council of Canada
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Made by gene synthesis
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PFA0445w-bio-His was a gift from Gavin Wright (Addgene plasmid # 50811 ; http://n2t.net/addgene:50811 ; RRID:Addgene_50811) -
For your References section:
Towards a comprehensive Plasmodium falciparum merozoite cell surface and secreted recombinant protein library. Zenonos ZA, Rayner JC, Wright GJ. Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93. 10.1186/1475-2875-13-93 PubMed 24620899