PF13_0125-bio-His
(Plasmid #50808)

• Purpose: Expresses enzymatically monobiotinylated full length PF13_0125
• Depositing Lab: Gavin Wright
• Publication: Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher
• Backbone size w/o insert (bp): 6670
• Modifications to backbone: Addition of CD4, biotinylation sequence, 6xhis tags
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PF13_0125
• Species: P. falciparum
• Insert Size (bp): 836
• Mutation: all potential N-linked glycosylation sites (N-X-S/T, where X is not proline) were systematically removed by substituting alanine for serine/threonine at these sites.
• Promoter: CMV
• Tag / Fusion Protein:
  • ratCD4d3+4,biotinylation sequence, 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBVori-F (5'-CCACTCAGTGTTGGCAAATG-3')

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Made by gene synthesis