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Addgene

pMXs-ms-Klf5
(Plasmid #50787)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50787 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXs-gw
  • Backbone manufacturer
    Yamanaka Lab, Addgene plasmid #18656
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Klf5
  • Alt name
    Kruppel-like factor 5, CKLF; IKLF; Bteb2; 4930520J07Rik
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1341
  • Entrez Gene
    Klf5 (a.k.a. 4930520J07Rik, Bteb2, CKLF, IKLF)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gateway cassette and pENTRE/D-TOPO are from Invitrogen Corporation. pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-ms-Klf5 was a gift from Shinya Yamanaka (Addgene plasmid # 50787 ; http://n2t.net/addgene:50787 ; RRID:Addgene_50787)

  • For your References section:

    Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, Okita K, Mochiduki Y, Takizawa N, Yamanaka S. Nat Biotechnol. 2008 Jan;26(1):101-6. Epub 2007 Nov 30. 10.1038/nbt1374 PubMed 18059259
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