pMXs-ms-Sox3
(Plasmid
#50779)
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Purposeretroviral expression of mouse Sox3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50779 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs-gw
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Backbone manufacturerYamanaka Lab, Addgene plasmid #18656
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSox3
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Alt nameSRY (sex determining region Y)-box 3
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Alt nameSox-3
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SpeciesM. musculus (mouse)
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Insert Size (bp)1353
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Entrez GeneSox3 (a.k.a. Sox-3)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pMX-S1811 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGateway cassette and pENTRE/D-TOPO are from Invitrogen Corporation. pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-ms-Sox3 was a gift from Shinya Yamanaka (Addgene plasmid # 50779 ; http://n2t.net/addgene:50779 ; RRID:Addgene_50779) -
For your References section:
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, Okita K, Mochiduki Y, Takizawa N, Yamanaka S. Nat Biotechnol. 2008 Jan;26(1):101-6. Epub 2007 Nov 30. 10.1038/nbt1374 PubMed 18059259