pMXs-ms-L-Myc-L351P
(Plasmid #50777)

• Purpose: retroviral expression of mouse L-Myc L351P mutant
• Depositing Lab: Shinya Yamanaka
• Publication: Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. ():.

Backbone

• Vector backbone: pMXs-gw
• Backbone manufacturer: Yamanaka Lab, Addgene plasmid #18656
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: L-Myc-L351P
• Alt name: v-myc myelocytomatosis viral oncogene homolog, lung carcinoma derived (avian), L-myc; Lmyc1; Mycl1; Lmyc-1; bHLHe38; AW536278
• Species: M. musculus (mouse)
• Insert Size (bp): 1320
• Mutation: L351P (does not bind to Max)
• Entrez Gene: Mycl (a.k.a. L-myc, Lmyc-1, Lmyc1, Mycl1, bHLHe38)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pMX-S1811

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gateway cassette and pENTRE/D-TOPO are from Invitrogen Corporation. pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry