pMXs-ms-c-Myc-V394D
(Plasmid #50775)

• Purpose: retroviral expression of mouse c-Myc V394D mutant
• Depositing Lab: Shinya Yamanaka
• Publication: Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. ():.

Backbone

• Vector backbone: pMXs-gw
• Backbone manufacturer: Yamanaka Lab, Addgene plasmid #18656
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: c-Myc V394D
• Alt name: v-myc avian myelocytomatosis viral oncogene homolog, MRTL; MYCC; c-Myc; bHLHe39
• Species: M. musculus (mouse)
• Insert Size (bp): 1320
• Mutation: V394D (does not bind to Miz-1)
• Entrez Gene: Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pMX-S1811

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gateway cassette and pENTRE/D-TOPO are from Invitrogen Corporation. pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry