pCYP119
(Plasmid
#50771)
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Purposeexpresses CYP 119 in e. coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC18
- Backbone size w/o insert (bp) 2600
- Total vector size (bp) 3800
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCYP119
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SpeciesSulfolobus solfatarius
- Promoter lac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eco R1 (not destroyed)
- 3′ cloning site Bam H1 (not destroyed)
- 5′ sequencing primer M13 reverce
- 3′ sequencing primer M13 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCYP119 was a gift from Stephen Sligar (Addgene plasmid # 50771 ; http://n2t.net/addgene:50771 ; RRID:Addgene_50771) -
For your References section:
Characterization of a cytochrome P450 from the acidothermophilic archaea Sulfolobus solfataricus. McLean MA, Maves SA, Weiss KE, Krepich S, Sligar SG. Biochem Biophys Res Commun. 1998 Nov 9;252(1):166-72. 10.1006/bbrc.1998.9584 PubMed 9813164