pEGFP-C1-hFGD5
(Plasmid
#50760)
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Purposeexpression of EGFP-tagged human FGD5
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50760 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFGD5
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SpeciesH. sapiens (human)
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Entrez GeneFGD5 (a.k.a. ZFYVE23)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-hFGD5 was a gift from Naoki Mochizuki (Addgene plasmid # 50760 ; http://n2t.net/addgene:50760 ; RRID:Addgene_50760) -
For your References section:
Rap1 potentiates endothelial cell junctions by spatially controlling myosin II activity and actin organization. Ando K, Fukuhara S, Moriya T, Obara Y, Nakahata N, Mochizuki N. J Cell Biol. 2013 Sep 16;202(6):901-16. doi: 10.1083/jcb.201301115. Epub 2013 Sep 9. 10.1083/jcb.201301115 PubMed 24019534